The 16s rDNA collection
February 9, 2009The last few weeks I have been working on the amplification of the 16s rDNA of many of the collected bioluminescent bacteria of the lab. It was the first time that I did a LARGE scale procedure. I am very proud that the results were positive and most of the reactions were held out successfully. I also prepared PCR’s that amplified the luxH region of the large stack of samples.
Results from the 16s rDNA amplicons :
By looking at these results I confirmed that the 16s rDNA reactions give me the DNA that I will need to work this semester with the restrictions enzymes and produce the elaborate results that I desire.
The next 4 pictures are the luxH amplicons of the 60 sample run:
