New Semester: Round 3

The work in this new semester has already begun. The amplification results of 16s rDNA and luxH have been sent to the United States in order to obtain a proper DNA sequence for each result. If the sequence is luxH or a 16s rDNA the correct conclusions can be designed. Also I’ve arranged a meeting with Josue Malavé, my grad-student mentor, with the purpose of establishing the next objectives for this semester.

The most important objectives for this semester are the following:

·                     Design the proper primers for the amplification of the luxAB region inside the lux gene. This region is responsible for the production of luciferase so in order to clone the whole gene, this region is of innermost importance.  After these primers are designed the next obstacle will be to find the most suiting parameters for a proper PCR. After this is done, the amplification of the other lux gene segments will be amplified following the same steps. As a reminder, the amplification of the different lux genes can be used for a proper molecular method of identification between bioluminescent bacteria amongst Puerto Rico’s coastlines.

·                   Perform ‘in vitro’ analyses using several programs that can help us decide the proper restriction enzymes that can undertake an ARDRA that discriminates amongst bioluminescent bacteria in Puerto Rico.

 

The work in this semester is the continuance of last semester’s experimental work.

 

Still, this semester, I will probably perform several experiments that will help us identify our samples with a molecular method that involves the primers that were designed on the last semester’s experiments.

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