RESULTS and FUTURE OBJECTIVES

The results from the last moths are no diferent. The experiments have been repeated and the amplification is occurring accurately and constantly. As for the luciferase genes, there are a lot of variations for each sample so the compilation and design of a universal set of primers that amplifies this region.  My next step will be to design then many primers (a less as possible) that amplify the region so the bacteria can be properly identified. It is important to recall that the less set of primers designed makes the method easier and shorter. Still the number of sets primers used will depend on how exact and reliable the results are.  I’m still waiting on the Photobacterium phosphoreum sample which will help me optimize the amplification of the luxF gene.