Looking at the Objective Checklist:

    This past month my work has been in silico, which means that Ive been doing some research and analysis at my computer. Although I had the advantage of working anywhere I wanted, some problems took place, but with time and practice I managed to understand how the program works. I used a gene data base called BLAST in which I managed to obtain all the sequences I wanted.

      When I had all the sequences that I wanted to study, I used another program to align them and therefore design the primer that I was going to use to amplify the area of the lux gene that interests or matters to the research.

I would say that my research deserves a 2 in progress because my first and one of the most important objective is basically covered and now i will proceed to verify if the method proposed works (which is my second objective: To do the tests in vivo). In the next few weeks I will inform on how things went out with the new set of primers.

I’ve encountered a few problems working with the programs in the design of primers, but these helped me to understand the concept completely and now I feel really comfortable on designing these.

See you soon.