New Semester: ROUND TWO!
August 17, 2008Fall 2008:
The first meetings have taken place. Security measures and other important rules have been discussed in these meetings and this week I will resume my work on the bio-marker. In the summer I read various papers and basically understood what Im going to do on this semester.
A brief summary:
I will develop a molecular tool which will help identify the bio luminescent bacteria in Puerto Rico’s coasts.
I will do it using ARDRA (amplified ribosomal DNA restriction analysis) methods in which the LUX genes of the bacteria will be amplified.
The LUX gene is basically the same on most bio luminescent bacteria except for some few details.
These small but important differences will be the key for my molecular tool.
My first step will be to design a primer so I can amplify the LUX genes so I have a lot of DNA to work with.
The techniques I used in the last semester were done because by that time I did not have the knowledge of basic Microbiology and Genetics. Those techniques helped me understand the pourpuse of the research I will undertake this semester.
As a reminder, these next weeks I will be designing a primer that amplifies the LUX F gene.
This gene will help me distinguish among different bioluminescent bacteria.
Then I will be finding the proper restriction enzymes. But, lets go ONE step at a time.
I will inform you the success or failure that I get in some time.
STAY TUNED!!!!
-Diego Hernandez Aranda
Los objetivos se ven excelente.Si puedes alcanzar los mismo (con dedicacion y compromiso)tendras buena data para presentar. Mucho exito!